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Your Position: Home > NAb kits > Spike RBD > RAS-N087

Anti-SARS-CoV-2 (BA.2) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD)

For research use only.
Materials Provided
IDComponentsSize
RAS087-C01Pre-coated Human ACE2 Microplate1 plate
RAS087-C02Positive Control100 μL
RAS087-C03Negative Control100 μL
RAS087-C04HRP-SARS-CoV-2 Spike RBD(BA.2)15 μg
RAS087-C0510xWashing Buffer50 mL
RAS087-C06Dilution Buffer50 mL
RAS087-C07Substrate Solution12 mL
RAS087-C08Stop Solution7 mL
  • Background
    A more contagious subvariant of omicron, known as BA.2, is spreading across the globe and could soon become the dominant version of Covid-19.It's now the top variant in at least 18 countries and rapidly spreading, representing 35% of all new cases that have been genetically sequenced worldwide, up from 10 countries and 21% of cases the week before, according to new data from the World Health Organization. In the U.S, BA.2 currently makes up 3.8% of genetically sequenced Covid cases, according to the Centers for Disease Control and Prevention.To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure neutralizing antibodies against the mutants is in urgent need.
  • Application
    The kit is developed for qualitative detection or titer measurement of Anti-SARS-CoV-2 (BA.2) neutralizing antibody (Spike RBD) in human serum.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit is used to measure the levels of Anti-SARS-CoV-2 neutralizing antibody through a competitive ELISA. The microplate in the kit has been pre-coated with Human ACE2 protein. First serum samples, Positive control,Negative Control are added to the wells followed by addition of HRP-SARS-CoV-2 Spike RBD. After incubation, the wells are washed and substrate is added to the wells. The reaction is terminated by the addition of stop solution and the intensity of color is measured at 450 nm. The presence of neutralizing antibodies in samples will compete with ACE2 for HRP-SARS-CoV-2 Spike RBD binding. The intensity of assay signal decrease proportionally to the presence of Anti-SARS-CoV-2 neutralizing antibody.

    Your experiment will include 5 simple steps:

    a) All reagents were returned to room temperature(20°C-25°C) before use.

    b) Make series the tested sample and control with ditlution buffer,HRP-SARS-CoV-2 RBD diluted with dilution buffer.

    c) Add the HRP-SARS-CoV-2 RBD ,diluted sample and Control add the plate respectively.

    d) Wash the plate and add TMB or other colorimetric HRP substrate.

    e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

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Price(USD) : $650.00

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