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| ID | Components | Size |
| EP133-C01 | High-bind Plate | 1 plate |
| EP133-C02 | Human PD-L1 | 10 μg |
| EP133-C03 | Biotinylated Human PD-1 | 30 μg |
| EP133-C04 | Anti-PD-L1 Neutralizing Antibody | 20 μg |
| EP133-C05 | Streptavidin-HRP | 10 μg |
| EP133-C06 | Coating Buffer | 12mL |
| EP133-C07 | 10xWashing Buffer | 50mL |
| EP133-C08 | Blocking Buffer | 50mL |
| EP133-C09 | Substrate Solution | 12mL |
| EP133-C10 | Stop Solution | 7mL |
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
INHIBITION OF PD-L1: PD-1[Biotinylated] BINDING BY ANTI-PD-L1 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-PD-L1 Neutralizing antibody (Catalog # EP133-C04) (1:1 serial dilution, from 2 μg/mL to 0.015625μg/mL) was added into PD-L1: Biotinylated PD-1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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