|EP138-C02||Human SIRP alpha||15μg|
|EP138-C03||Biotinylated Human CD47||10μg|
|EP138-C04||Anti-CD47 Neutralizing Antibody||20μg|
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human SIRP alpha.
b) Add your molecule of interest to the tests.
c) Add human CD47-Biotin to bind the coated SIRP alpha.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to CD47: SIRP alpha binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF CD47[Biotinylated]: SIRP alpha BINDING BY ANTI-CD47 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-CD47 Neutralizing antibody (Catalog # EP138-C04) (1:1 serial dilution, from 10 μg/mL to 0.01μg/mL) was added into Biotinylated CD47: SIRP alpha binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $650.00
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