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Your Position: Home > Kits > CD47 > EP-138

CD47[Biotinylated] : SIRP alpha Inhibitor Screening ELISA Kit

For research use only.

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EP138-C01High-bind Plate1plate
EP138-C02Human SIRP alpha15μg
EP138-C03Biotinylated Human CD4710μg
EP138-C04Anti-CD47 Neutralizing Antibody20μg
EP138-C06Coating Buffer12mL
EP138-C0710xWashing Buffer 50mL
EP138-C08Blocking Buffer50mL
EP138-C09Substrate Solution12mL
EP138-C10Stop Solution12mL
  • Background
    CD47 is a transmembrane protein, a cell surface glycoprotein molecule belonging to the immunoglobulin superfamily that binds to a variety of proteins, including integrins, thrombospondin 1, and signal regulatory protein-α (SIRPα). In recent years, accumulating data have demonstrated that the CD47-SIRP-α axis is a key immune checkpoint in different cancers, including hematological malignancies, similar to PD-1/PD-L1 in solid tumors. Cd47-sirpα blockade has become a next-generation immune checkpoint blocking strategy for various malignancies after PD-1/PD-L1.
  • Application
    This kit is developed for screening for inhibitors of human CD47 binding to human SIRP alpha.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new CD47 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human CD47 to immobilized human SIRP alpha in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated CD47 protein, a human SIRP alpha protein, an anti-CD47 neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

    Your experiment will include 4 simple steps:

    a) Coat the plate with human SIRP alpha.

    b) Add your molecule of interest to the tests.

    c) Add human CD47-Biotin to bind the coated SIRP alpha.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the half maximal inhibitory concentration (IC50) of your compound to CD47: SIRP alpha binding will be determined by comparing OD readings among different experimental groups.

Typical Data Please refer to DS document for The assay protocol.

Serial dilutions of Anti-CD47 Neutralizing antibody (Catalog # EP138-C04) (1:1 serial dilution, from 10 μg/mL to 0.01μg/mL) was added into Biotinylated CD47: SIRP alpha binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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ETD of in-stock products: 2 business days

Price(USD) : $650.00

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