|EP143-C01||High-bind Plate||1 plate|
|EP143-C02||Human TNFR1||15 μg|
|EP143-C03||Biotinylated Human TNF-alpha||10 μg|
|EP143-C04||Anti-TNF-alpha Neutralizing Antibody||20 μg|
|EP143-C06||Coating Buffer||12 mL|
|EP143-C07||10xWashing Buffer||50 mL|
|EP143-C08||Blocking Buffer||50 mL|
|EP143-C09||Substrate Solution||12 mL|
|EP143-C10||Stop Solution||7 mL|
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human TNFR1 .
b) Add your molecule of interest to the tests.
c) Add human TNF-alpha-Biotin to bind the coated TNFR1.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to TNF-alpha: TNFR1 binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF TNF-alpha [Biotinylated]: TNFR1 BINDING BY ANTI- TNF-alpha NEUTRALIZING ANTIBODY
Serial dilutions of Anti-TNF-alpha Neutralizing antibody (Catalog # EP143-C04) (1:1 serial dilution, from 10 μg/mL to 0.005μg/mL) was added into Biotinylated TNF-alpha: TNFR1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $650.00
ComboX™ is a new brand of ACROBiosystems. ComboX™ products include our Streptavidin series products, coated plates, isotype controls for antibody and fusion protein drug research, and general-purpose antibodies.
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