|EP145-C01||High-bind Plate||1 plate|
|EP145-C02||Human TNFSF11||15 μg|
|EP145-C03||HRP-Human Osteoprotegerin||10 μg|
|EP145-C04||Anti-TNFSF11 Neutralizing Antibody||20 μg|
|EP145-C05||Coating Buffer||12 mL|
|EP145-C06||10xWashing Buffer||50 mL|
|EP145-C07||Blocking Buffer||50 mL|
|EP145-C08||Substrate Solution||12 mL|
|EP145-C09||Stop Solution||7 mL|
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human TNFSF11.
b) Add your molecule of interest to the tests.
c) Add human Osteoprotegerin to bind the coated TNFSF11.
d) Add TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to TNFSF11: Osteoprotegerin binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF TNFSF11: Osteoprotegerin BINDING BY ANTI-TNFSF11 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-TNFSF11 Neutralizing antibody (Catalog # EP145-C04) (1:1 serial dilution, from 20 μg/mL to 0.01μg/mL) was added into TNFSF11: Osteoprotegerin binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $650.00
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