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Your Position: Home > Immune Checkpoint Proteins > CD47 & SIRP alpha > Human CD47 Protein

Human CD47 Protein

  • Synonym
    CD47,MER6,IAP,OA3
  • Source
    Human CD47, His Tag (CD7-H5227) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # NP_942088).
    Predicted N-terminus: Gln 19
  • Molecular Characterization
    Online(Gln 19 - Pro 139) NP_942088

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 15.6 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD47, His Tag (Cat. No. CD7-H5227) SDS-PAGE gel

Human CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human CD47, His TagHuman CD47, His Tag (Cat. No. CD7-H5227) ELISA bioactivity

Immobilized Human SIRP alpha, Fc Tag (Cat. No. SIA-H5251) at 5 μg/mL (100 μL/well) can bind Human CD47, His Tag (Cat. No. CD7-H5227) with a linear range of 2-13 ng/mL (QC tested).

Human CD47, His TagHuman CD47, His Tag (Cat. No. CD7-H5227) ELISA bioactivity

Immobilized Anti-CD47 MAb, Human IgG4 (clone 5F9) at 2 μg/mL (100 μL/well) can bind Human CD47, His Tag (Cat. No. CD7-H5227) with a linear range of 0.4-3 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-Human CD47 MAb (Human IgG4) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human CD47, His Tag (Cat. No. CD7-H5227) with an affinity constant of 1.66 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

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Price(USD) : $280.00

Price(USD) : $2100.00

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CD47 & SIRP alpha Products

  • Name:Cluster of Differentiation 47
  • Classification:Homo sapiens
  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:11 Details
  • Lastest Research Phase:Phase ?
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