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Your Position: Home > Bio-Markers & CD Antigens > TNFR1 > Human TNFR1 CD120a TNFRSF1A Protein, With C-Fc Tag

Human TNFR1 / CD120a / TNFRSF1A Protein, Fc Tag

  • Synonym
    TNFR1,CD120a,TNFRSF1A,FPF,TBP1,TNF-R,TNF-R-I,TNF-R55,TNFAR,TNFR55,TNFR60,p55,p60
  • Source
    Human TNFR1, Fc Tag (TN1-H5251) is expressed from human 293 cells (HEK293). It contains AA Ile 22 - Thr 211 (Accession # NP_001056).
    Predicted N-terminus: Ile 22
  • Molecular Characterization
    TNFR1(Ile 22 - Thr 211) NP_001056

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 47.9 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >98% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human TNFR1, Fc Tag (Cat. No. TN1-H5251) SDS-PAGE gel

Human TNFR1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity
Human TNFR1, Fc TagHuman TNFR1, Fc Tag (Cat. No. TN1-H5251) ELISA bioactivity

Immobilized ActiveMax® Human TNF-alpha (Cat. No. TNA-H4211) at 5 µg/mL (100 µl/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.04-1.2 ng/mL (QC tested).

  • Background
    Tumor necrosis factor receptor 1 (TNF-R1) is also known as Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), TNFAR, CD antigen CD120a, which belongs to the tumor necrosis factor receptor superfamily. TNF-R1 contains one death domain and four TNFR-Cys repeats. TNF-R1 is the receptor of TNFSF2 / TNF-alpha and homotrimeric TNFSF1 / lymphotoxin - alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TNF-R1 contributes to the induction of non - cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Defects in TNFRSF1A are the cause of familial hibernian fever (FHF).
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $250.00

Price(USD) : $1600.00

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