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| Assay Type | Sandwich-ELISA |
| Analyte | Soluble TROP-2 |
| Format | 96T |
| Reactivity | Human |
| Sensitivity | 46.875 pg/mL |
| Standard Curve Range | 46.875 pg/mL-12000 pg/mL |
| Assay Time | 1 hr 45 min |
| Suitable Sample Type | For the quantitative determination of human Soluble TROP-2 in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 μL |
| ID | Components | Size |
| CEA032-B01 | Pre-coated Anti-TROP-2 Antibody Microplate | 1 plate |
| CEA032-B02 | Human TROP-2 Standard | 20 μg ×2 |
| CEA032-B03 | Biotin-Anti-TROP-2 Antibody Con. Solution | 400 μL |
| CEA032-B04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA032-B05 | Streptavidin-HRP Con. Solution | 0.5 mL |
| CEA032-B06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA032-B07 | 20× Washing Buffer | 50 mL |
| CEA032-B08 | Sample Dilution Buffer | 15 mL ×2 |
| CEA032-B09 | Substrate Solution | 12 mL |
| CEA032-B10 | Stop Solution | 6 mL |
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
The kit is developed for quantitative detection of human TROP2 in serum, plasma and cell culture supernates.
It is for research use only.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CEA032-B02 is less than 46.875 pg/mL.
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