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Your Position: Home > Kits > NKp46 > CEA-B040

ClinMax™ Human NKp46/NCR1 ELISA Kit, PRO

For research use only.

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Product Details
Assay TypeSandwich-ELISA
AnalyteNKp46
Format96T
ReactivityHuman
Sensitivity15.0 pg/mL
Standard Curve Range15.625 pg/mL-1000 pg/mL
Assay Time2 hr 45 min
Suitable Sample TypeFor the quantitative determination of human NKp46 in Cell Culture Supernatants, Plasma, Serum.
Sample volume100 μL
Materials Provided
IDComponentsSize
CEA040-B01Pre-coated Anti-NKp46 Antibody Microplate1 plate
CEA040-B02Human NKp46 Standard20 μg ×2
CEA040-B03Biotin-Anti-NKp46 Antibody Con. Solution200 μL
CEA040-B042×Biotin-Antibody Dilution Buffer8mL
CEA040-B05Streptavidin-HRP Con. Solution0.5 mL×2
CEA040-B06Streptavidin-HRP Dilution Buffer15 mL
CEA040-B0720× Washing Buffer50 mL
CEA040-B08Sample Dilution Buffer15 mL ×2
CEA040-B09Substrate Solution12 mL
CEA040-B10Stop Solution6 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human NKp46/NCR1 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of human NKp46 in serum, plasma and cell culture supernates.

    It is for research use only.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
  • Assay Principles
     NKp46 Assay Principles
Typical Data Please refer to DS document for the assay protocol.
 NKp46 TYPICAL DATA

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CEA040-B02 is less than 15.0 pg/mL.

  • Background: NKp46
    Natural cytotoxicity triggering receptor 1 (NCR1) is also known as Natural killer cell p46-related protein (NK-p46), Lymphocyte antigen 94 homolog (LY94), CD antigen CD335, which belongs to the natural cytotoxicity receptor (NCR) family. NCR1 contains two Ig-like (immunoglobulin-like) domains. NCR1 interacts with CD247 and FCER1G. NCR1 / CD335 may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
  • Clinical and Translational Updates

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