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Your Position: Home > Kits > GDF15 > EP-164

GDF15 [Biotinylated] : GFRAL Inhibitor Screening ELISA Kit

For research use only.
EP164-C01High-bind Plate1 plate
EP164-C02Human GFRAL10 μg
EP164-C03Biotinylated Human GDF155 μg
EP164-C04Anti-GDF15 Neutralizing Antibody10 μg
EP164-C05Streptavidin-HRP5 μg
EP164-C06Coating Buffer12 mL
EP164-C0720xWashing Buffer 50 mL
EP164-C08Blocking Buffer50 mL
EP164-C09Substrate Solution12 mL
EP164-C10Stop Solution7 mL
  • Background
    Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1). Expression of MIC-1 mRNA in monocytoid cells is up-regulated by a variety of stimuli associated with activation, including interleukin 1β, tumor necrosis factor α (TNF-α), interleukin 2, and macrophage colony-stimulating factor but not interferon γ, or lipopolysaccharide (LPS). It is highly expressed in cardiomyocytes, adipocytes, macrophages, endothelial cells, and vascular smooth muscle cells in normal and pathological condition. GDF-15 increases during tissue injury and inflammatory states and is associated with cardiometabolic risk. Increased GDF-15 levels are associated with cardiovascular diseases such as hypertrophy, heart failure, atherosclerosis, endothelial dysfunction, obesity, insulin resistance, diabetes, and chronic kidney diseases in diabetes. Increased GDF-15 level is linked with the progression and prognosis of the disease condition.
  • Application

    This kit is developed for screening for inhibitors of human GDF-15 binding to human GFR alpha-like.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 64 days from the date of opening.
  • Assay Principles
    This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new OX40 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human OX40 to immobilized human OX40 Ligand in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated OX40 protein, a human OX40 Ligand protein, an anti-OX40 neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

    Your experiment will include 4 simple steps:

    a) Coat the plate with human OX40 Ligand.

    b) Add your molecule of interest to the tests.

    c) Add Biotinylated Human OX40 to bind the coated OX40 Ligand.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the half maximal inhibitory concentration (IC50) of your compound to OX40 : OX40 Ligand binding will be determined by comparing OD readings among different experimental groups.

Typical Data Please refer to DS document for The assay protocol.

Serial dilutions of Anti-GDF15 Neutralizing Antibody (Catalog # EP164-C04) (1:1 serial dilution, from 2 μg/mL to 0.000976563μg/mL) was added into GDF15 [Biotinylated]: GFRAL binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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ETD of in-stock products: 2 business days

Price(USD) : $650.00

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