Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Kits > DLL4 > CEA-B038

ClinMax™ Human Soluble Delta Like Protein 4 (DLL4) ELISA Kit, PRO

For research use only.

Order Now

Product Details
Assay TypeSandwich-ELISA
AnalyteSoluble Delta Like Protein 4 (DLL4)
Format96T
ReactivityHuman
Regulatory StatusPRO
Sensitivity5.0 pg/mL
Standard Curve Range15.625 pg/mL-1000 pg/Ml
Assay Time2 hr
Suitable Sample TypeFor the quantitative determination of human Soluble Delta Like Protein 4 (DLL4) in Cell Culture Supernatants, Plasma, Serum.
Sample volume50 μL
Materials Provided
IDComponentsSize
CEA038-B01Pre-coated Anti-DLL4 Antibody Microplate1 plate
CEA038-B02Human DLL4 Standard100 μg ×2
CEA038-B03Biotin-Anti-DLL4 Antibody Con. Solution400 μL
CEA038-B04Biotin-Antibody Dilution Buffer8mL
CEA038-B05Streptavidin-HRP Con. Solution500 μL
CEA038-B06Streptavidin-HRP Dilution Buffer15 mL
CEA038-B0720× Washing Buffer50 mL
CEA038-B08Sample Dilution Buffer15 mL ×2
CEA038-B09Substrate Solution12 mL
CEA038-B10Stop Solution6 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human Delta Like Protein 4 (DLL4) that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of human DLL4 in serum, plasma and cell culture supernates.

    It is for research use only.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
  • Assay Principles
     DLL4 Assay Principles
Typical Data Please refer to Ds document for the assay protocol.
 DLL4 TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of DLL4 is less than 5.0 pg/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 4 samples containing different DLL4 concentrations were tested in one assay. Acceptable criteria: CV<10%.

 DLL4 INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Five samples containing different concentrations of DLL4 were tested in independent assays. Acceptable criteria: CV<15%.

 DLL4 INTER-ASSAY STATISTICS
Recovery

Recombinant DLL4 was spiked into 3 human serum samples, and then analyzed. The average recovery of DLL4 for serum samples is 89.2%.

 DLL4 RECOVERY
  • Background: DLL4
    Delta-like protein 4 (DLL4) is also known as Drosophila Delta homolog 4 (Delta4), which contains one DSL domain and eight EGF-like domains. DLL4 is expressed in vascular endothelium. DLL4 is involved in the Notch signaling pathway as Notch ligand, which can activates NOTCH1 and NOTCH4. DLL4 is involved in angiogenesis and negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. DLL4 can bind to Notch-1 and Notch-4.
  • Clinical and Translational Updates

Comments (0)


ETA of in-stock products: 2 business days

Price(USD) : $590.00

Price(USD) :


Promotion & Exhibitions



Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:3 Details
  • Latest Research Phase:Phase 3 Clinical

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message