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TNF-alpha : TNFR1[HRP] Inhibitor Screening ELISA Kit-Pre coated plate

For research use only.

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    Materials Provided
    IDComponentsSize
    EP165-C01Human TNF-alpha coated Plate1 plate
    EP165-C02Anti-TNF-alpha Neutralizing Antibody10 μg
    EP165-C03HRP-Human TNFR110 μg
    EP165-C0420xWashing Buffer 50 mL
    EP165-C051×Dilution Buffer50 mL
    EP165-C06Substrate Solution12 mL
    EP165-C07Stop Solution7 mL
  • Product Overview
    TNF-alpha : TNFR1[HRP] Inhibitor Screening ELISA Kit-Pre coated plate is based on a competition-ELISA method, is used to screening for inhibitors of human TNF-alpha binding to human TNFR1. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human TNF-alpha binding to human TNFR1.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    Unopened kit should be stored at 2°C -8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Typical Data Please refer to DS document for the assay protocol.
     TNF-alpha TYPICAL DATA

    INHIBITION OF TNF-alpha: TNFR1[HRP] BINDING BY ANTI- TNF-alpha NEUTRALIZING ANTIBODY
    Serial dilutions of ANTI- TNF-alpha Neutralizing Antibody (Catalog # EP165-C02) (1:1 serial dilution, from 5μg/mL to 0.002441406 μg/mL) was added into TNF-alpha: TNFR1[HRP] binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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