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Your Position: Home > Protein > SLAMF7 > SL7-HP2H6

PE-Labeled Human SLAMF7 / CRACC / CD319 Protein, His TagStar Staining

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  • Synonym
    SLAMF7,CD319,CS1,CRACC,19A,FOAP-12
  • Source
    PE-Labeled Human SLAMF7 Protein, His Tag (SL7-HP2H6) is produced via conjugation of PE to Human SLAMF7 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human SLAMF7 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Met 226 (Accession # AAH27867).
    Predicted N-terminus: Ser 23
  • Molecular Characterization
    SLAMF7 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 36.9 kDa.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Evaluation of CAR expression
FACS Analysis of Anti-SLAMF7 CAR Expression
 SLAMF7 CAR_T

5e5 of anti-SLAMF7/CS1 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human SLAMF7 Protein, His Tag (Cat. No. SL7-HP2H6) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). PE signal was used to evaluate the binding activity (QC tested).

FACS Analysis of Non-specific binding to PBMCs
 SLAMF7 CAR_T

5e5 of PBMCs were stained with PE-Labeled Human SLAMF7 Protein, His Tag (Cat. No. SL7-HP2H6) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).

  • Background
    SLAM family member 7 (SLAMF7) is also known as CD2-like receptor-activating cytotoxic cells (CRACC), Membrane protein FOAP-12, CD antigen CD319, Novel Ly9, Protein 19A, which is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Isoform 1 of SLAMF7 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. May play a role in lymphocyte adhesion. Isoform 3 of SLAMF7 does not mediate any NK cell activation.
  • Clinical and Translational Updates

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