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Your Position: Home > Kits > BCMA > CEA-B045

ClinMax™ Human Soluble BCMA ELISA Kit

For research use only.

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Product Details
Assay TypeSandwich-ELISA
AnalyteSoluble BCMA
Format96T
ReactivityHuman
Sensitivity150 pg/mL
Standard Curve Range375 pg/mL-24000 pg/mL
Assay Time2 hr 45 min
Suitable Sample TypeFor the quantitative determination of human Soluble BCMA in Cell Culture Supernatants, Plasma, Serum.
Sample volume50 uL
Materials Provided
IDComponentsSize
CEA045-C01Pre-coated Anti-Soluble BCMA Antibody Microplate1 plate
CEA045-C02Human Soluble BCMA Standard320 μg×2
CEA045-C03Biotin-Anti-Soluble BCMA Antibody Con. Solution100 μL
CEA045-C04Biotin-Antibody Dilution Buffer8 mL
CEA045-C05Streptavidin-HRP Con. Solution500 μL
CEA045-C06Streptavidin-HRP Dilution Buffer15 mL
CEA045-C0720× Washing Buffer50 mL
CEA045-C08Sample Dilution Buffer15 mL×2
CEA045-C09Substrate Solution12 mL
CEA045-C10Stop Solution6 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human soluble BCMA that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-like facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of human soluble BCMA in serum, plasma and cell culture supernates.

    It is for research use only.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
  • Assay Principles
     BCMA Assay Principles
Typical Data Please refer to DS document for the assay protocol.
 BCMA TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of BCMA is less than 150 pg/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 3 samples containing different BCMA concentrations were tested in one assay. Acceptable criteria: CV<10%.

 BCMA INTRA-ASSAY STATISTICS
Inter-Assay Statistics

3 samples containing different concentrations of BCMA were tested in independent assays. Acceptable criteria: CV<15%.

 BCMA INTER-ASSAY STATISTICS
Recovery

Recombinant BCMA was spiked into 3 human serum samples, and then analyzed. The average recovery of BCMA for serum samples is 97.4%.

 BCMA RECOVERY
  • Background: BCMA
    Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.
  • Clinical and Translational Updates

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