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Ready-to-use Human iPSC-Derived Intestine Organoids

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  • Product Details
    Human iPSC-Derived Intestine Organoids are differentiated from hESC or iPSCs using Human iPSC-Derived Intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K). Intestine organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative to the human intestine regions. The Ready-to-use intestine organoids are derived form human iPS cells and being cultured for more than 60 days. Organoids generated using Human iPSC-Derived intestine Organoid feature various types of cells, including intestine epithelium cells, mesenchyme cells, enterocytes, Paneth cells, goblet cells, etc. These intestine organoids show intestine crypt like structure, Villi and Microvilli like structure. These intestine organoids show regular peristalsis and normal intestinal function validated by the absorption of fatty acid and glucose.
  • Product Specification
    The live organoids are ready-to-use organoids that are delivered in shipping medium and has to go through a 48h recovery process according to instruction.
  • Storage

    After recovery, please store the organoid in its maintenance medium under the correct incubation condition and medium changing process.

Product Diagram
 - PRODUCT DIAGRAM

Protocol Diagram of intestine organoid differentiation.

Validation Data
Organoid Histology and State
 - Organoid Histology And State

The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show regular peristalsis.

Orgnaoid Histology
 - ORGNAOID HISTOLOGY

Observation of granulocyte like cells, adipocyte like cells, smooth muscle like cells and intestinal crypt like structure by morphology on day 120 intestine organoids.

Marker Expression
 - MARKER EXPRESSION

The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of goblet cells (mucus-producing, MUC2), brush borders (Villin) and endothelial cells (CD31).

 - MARKER EXPRESSION

The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of enterochromaffin cells (CHGA) and endothelial cells (CD31).

 - MARKER EXPRESSION

The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of goblet cells (mucus-producing, MUC2) and endothelial cells (CD31).

 - MARKER EXPRESSION

The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of smooth muscle cell (α-SMA); epithelial cell (EPCAM); macrophage (CD68); goblet cell (MUC2); Ki67 (intestinal stem cell) and enteroendocrine cells (CHGA).

Organoid Activity
 - ORGANOID ACTIVITY

Intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show normal intestinal function validated by the absorption of fatty acid and glucose.

  • Clinical and Translational Updates

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