5e5 of anti-uPAR CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human uPAR, His Tag (Cat. No. UPR-HP2H7) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).
Mouse uPAR, His Tag (Cat. No. UPR-M52H3) immobilized on CM5 Chip can bind Human PLAU, His Tag (Cat. No. PLU-H5229) with an affinity constant of 14.1 nM as determined in a SPR assay (Biacore 8K) (QC tested).
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