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Rituximab

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Part of Bioactivity data

RIB-FY35c-Cell-based assay
FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-CD20 (Rituximab) CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Anti-Rituximab Antibodies, Mouse IgG1 (Cat. No. RIB-FY35c) and isotype control antibody respectively. FITC signal was used to evaluate the binding activity (QC tested).

RIB-Y36-Cell-based assay
FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that the binding of rituximab to 293F overexpressing CD20 was inhibited by increasing concentration of Anti-Rituximab Antibodies (Cat. No. RIB-Y36). The concentration of rituximab used is 10 ng/ml. The IC50 is 0.025 μg/ml (Routinely tested).

RIB-Y36-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-Rituximab Antibodies (mouse IgG1, Cat. No. RIB-Y36) captured on CM5 chip via anti-mouse antibodies surface, can bind human rituximab with an affinity constant of 0.01 nM.

RIB-FY35c-ELISA
FITC-Labeled Anti-Rituximab Antibodies, Mouse IgG1FITC-Labeled Anti-Rituximab Antibodies, Mouse IgG1 (Cat. No. RIB-FY35c) ELISA bioactivity

Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind FITC-Labeled Anti-Rituximab Antibodies, Mouse IgG1 (Cat. No. RIB-FY35c) with a linear range of 0.04-0.6 μg/mL (QC tested).

Synonym Name

Background

Rituxan is a genetically engineered chimeric murine/human monoclonal antibody directed against the CD20 antigen found on the surface of normal and malignant B lymphocytes. The antibody is an IgG1 kappa immunoglobulin containing murine light- and heavy-chain variable region sequences and human constant region sequences. Rituximab is composed of two heavy chains of 451 amino acids and two light chains of 213 amino acids

Clinical and Translational Updates

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