|EP101-C01||High-bind Plate||1 plate|
|EP101-C02||Human PD-L1||25 μg|
|EP101-C03||Anti-PD-1 Neutralizing Antibody||20 μg|
|EP101-C04||Human PD-1-Biotin||5 μg|
|EP101-C06||Coating Buffer||12 mL|
|EP101-C07||10xWashing Buffer||50 mL|
|EP101-C08||Blocking Buffer||50 mL|
|EP101-C09||Substrate Solution||12 mL|
|EP101-C10||Stop Solution||7 mL|
It is for research use only.
Your experiment will include 4 simple steps:
a) Coat the plate with human PD-L1.
b) Add your molecule of interest to the tests.
c) Add human PD-1-Biotin to bind the coated human PD-L1.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit PD-1 : PD-L1 binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF PD-1-PD-L1 BINDING BY ANTI-PD-1 NEUTRALIZING ANTIBODY (CATALOG # EP101-C03) MEASURED USING THE PD-1 [BIOTINYLATED] : PD-L1 INHIBITOR SCREENING ELISA ASSAY PAIR (CATALOG # EP-101).
Anti-PD-1 neutralizing antibody was diluted from 10 μg/mL to 0.078 μg/ mL (69.628 nM to 0.544 nM) and loaded onto the plate coated by human PD-L1 in the presence of human PD-1-Biotin. Assay was performed according to the protocol in PD-1[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $600.00
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