Human IL-12A / NKSF1 / p35 Protein, Fc Tag

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Cat. No. / Size
Price
Qty
NK1-H5252-50ug
$260.00
NK1-H5252-500ug
$1245.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    IL12A, CLMF, IL-12A, NFSK, NKSF1, P35

  • Source

    Human IL-12A, Fc Tag (NK1-H5252) is expressed from human 293 cells (HEK293). It contains AA Arg 23 - Ser 219 (Accession # P29459).

    Predicted N-terminus: Arg 23

    Request for sequence
  • Molecular Characterization

    IL-12A Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 49.2 kDa. The protein migrates as 57-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-12A SDS-PAGE

    Human IL-12A, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

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Background

Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF) , is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL12 is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th0 cells and plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. Interleukin-12 subunit alpha (IL12A) also known as NKSF1, CLMF1 and P35, IL12A shows significant sequence similarity to IL-6, G-CSF, and exerts biological activities only when the IL12B is co-expressed. IL12B deficient mice are resistant to the induction of experimental chronic inflammatory diseases whereas IL12A knock-out mice develop more severe forms, suggesting opposite functions of the two subunits in the outcome of chronic inflammatory diseases.

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Drug Development Progress
  • English Name:

    Interleukin-12 subunit alpha

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    0 Details

  • Highest Development Stage:

    Discontinued

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