Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
user
  1. Dashboard
  2. My Profile
  3. My Address
  4. My Orders
  5. Acro Membership
Login Register
Recombinant Proteins ClearClear history
Recombinant Proteins CloseClose
Browse Products By Molecule Name a-zA-Z
TL1A Hot |GMP IL-2 Hot |Streptavidin（SA） Hot |Claudin 18.2 |Cadherin-17 
0
There is no goods in the shopping cart !
My Cart|Check Out
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Protein > Her2 > HE2-H822R

Biotinylated Human Her2 / ErbB2 Protein, His Tag, ultra sensitivity (primary amine labeling, long spacer)

Recommended Hot Products:
SP-11 Streptavidin Coated Plates  Request a free sample 
STN-NH913 Streptavidin-HRP 
SMB-B01 Magnetic Beads™ Streptavidin 

Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.

Order Now

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    ERBB2,CD340,HER-2,neu,HER2,MLN19,NEU,NGL,TKR1
  • Source
    MABSol® Biotinylated Human Her2, His Tag, primary amine labeling, long spacer (HE2-H822R) is expressed from human HEK293 cells. It contains AA Thr 23 - Thr 652 (Accession # P04626-1 ). It is the biotinylated form of Human Her2, His Tag (Cat. No. HE2-H5225).
    Predicted N-terminus: Thr 23
    Request for sequence
  • Molecular Characterization
    Her2 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 70.2 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A specially optimized long-spacer biotin reagent (32.5 angstroms) is used in this product to minimize potential steric hindrance.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Her2 SDS-PAGE

Biotinylated Human Her2, His Tag, primary amine labeling, long spacer on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Her2 ELISA

Immobilized Trastuzumab at 3 μg/mL (100 μL/well) can bind Biotinylated Human Her2, His Tag, primary amine labeling, long spacer (Cat. No. HE2-H822R) with a linear range of 0.1-3.1 ng/mL (QC tested).

Protocol
Her2 BATCH ELISA
Report
Bioactivity-BLI
Her2 BLI

Immobilized Herceptin on SA Biosensor via Biotinylated Human Her2, His Tag, primary amine labeling, long spacer (Cat. No. HE2-H822R), can bind Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H5286) with an affinity constant of 1.32 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol
Bioactivity-FACS
Her2 FACS

2e5 of Anti-Her2 CAR-293 cells were stained with 100 μL of 1μg/mL of Biotinylated Human Her2, His Tag, primary amine labeling, long spacer (Cat. No. HE2-H822R) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

Protocol
  • Background
    Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .
  • Clinical and Translational Updates

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)


ETD of in-stock products: 2 business days

Price(USD) : $335.00

Price(USD) : $1586.00

Add to Cart
Bulk Inquiry Customize Product
Recommended Products
Streptavidin Coated Plates

Price(USD) : $32.00

Price(USD) : $137.00

Streptavidin-HRP

Price(USD) : $24.00

Price(USD) : $180.00

Magnetic Beads™ Streptavidin

Price(USD) : $138.00

Price(USD) : $230.00

Add to Cart
Bulk Inquiry Customize Product

Licensing

more

Promotion & Exhibitions



Drug Development Status

  • Number of Launched Drugs：30 Details
  • Number of Drugs in Clinical Trials：182 Details
  • Latest Research Phase：Approved
More Recommended Products

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)
Show All Distributors

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
Email
Email
order@acrobiosystems.com
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA
linkedin twitter facebook youtube

Leave a message

Copyright © 2017 ACROBiosystems. All Rights Reserved
|Related Links|Privacy Policy|Terms and Conditions|Legal|Sitemap