This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 70.2 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by reduced SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human Her2, His Tag, primary amine labeling, long spacer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Herceptin on SA Biosensor via Biotinylated Human Her2, His Tag, primary amine labeling, long spacer (Cat. No. HE2-H822R), can bind Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H5286) with an affinity constant of 1.32 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
2e5 of Anti-Her2 CAR-293 cells were stained with 100 μL of 1μg/mL of Biotinylated Human Her2, His Tag, primary amine labeling, long spacer (Cat. No. HE2-H822R) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Authors: Vivier D, et al
Journal: J NUCL MED 2019
Authors: Mortensen MR, et al.
Journal: Bioconjug Chem 2018
Authors: Kim Young Min, et al.
Journal: US20160136300A1 2016
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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