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Your Position: Home > Receptor Tyrosine Kinases > Her2 > HE2-H822R

Biotinylated Human Her2 / ErbB2 Protein, His Tag, ultra sensitivity (primary amine labeling, long spacer)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    ERBB2,CD340,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1
  • Source
    MABSol® Biotinylated Human Her2, His Tag, primary amine labeling, long spacer (HE2-H822R) is expressed from human HEK293 cells. It contains AA Thr 23 - Thr 652 (Accession # P04626-1). It is the biotinylated form of Human Her2, His Tag (Cat. No. HE2-H5225).
    Predicted N-terminus: Thr 23
    Request for sequence
  • Molecular Characterization
    Online(Thr 23 - Thr 652) P04626-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 70.2 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A specially optimized long-spacer biotin reagent (32.5 angstroms) is used in this product to minimize potential steric hindrance.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 1.5-3.5 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Her2, His Tag, primary amine labeling, long spacer (Cat. No. HE2-H822R) SDS-PAGE gel

Biotinylated Human Her2, His Tag, primary amine labeling, long spacer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human Her2, His Tag, primary amine labeling, long spacerBiotinylated Human Her2, His Tag, primary amine labeling, long spacer (Cat. No. HE2-H822R) ELISA bioactivity

Immobilized Trastuzumab at 3 μg/mL (100 μL/well) can bind Biotinylated Human Her2, His Tag, primary amine labeling, long spacer (Cat. No. HE2-H822R) with a linear range of 0.1-3.1 ng/mL (QC tested).

Protocol
Biotinylated Human Her2, His Tag, primary amine labeling, long spacerBiotinylated Human Her2, His Tag, primary amine labeling, long spacer (Cat. No. HE2-H822R) ELISA bioactivity
Report
Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Immobilized Herceptin on SA Biosensor via Biotinylated Human Her2, His Tag, primary amine labeling, long spacer (Cat. No. HE2-H822R), can bind Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H5286) with an affinity constant of 1.32 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol
Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-Her2 CAR-293 cells were stained with 100 μL of 1μg/mL of Biotinylated Human Her2, His Tag, primary amine labeling, long spacer (Cat. No. HE2-H822R) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

Protocol
  • Citations
  • Background
    Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .
  • References

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $1375.00

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