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Your Position: Home > Immune Checkpoint Proteins > CTLA-4 > Biotinylated Human CTLA-4 Protein, Fc,Avitag

Biotinylated Human CTLA-4 Protein, Fc,Avitag™

  • Synonym
    CTLA4,CD152
  • Source
    Biotinylated Human CTLA-4, Fc,Avitag (CT4-H82F3) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # P16410-1).
    Predicted N-terminus: Ala 37
  • Molecular Characterization
    Online(Ala 37 - Phe 162) P16410-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 41.8 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) SDS-PAGE gel

Biotinylated Human CTLA-4, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human CTLA-4, Fc,AvitagBiotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) ELISA bioactivity

Immobilized Human B7-2, Fc Tag (Cat. No. CD6-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) with a linear range of 0.6-10 ng/mL (QC tested).

Biotinylated Human CTLA-4, Fc,AvitagBiotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) ELISA bioactivity

Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) with a linear range of 0.6-10 ng/mL (Routinely tested).

Biotinylated Human CTLA-4, Fc,AvitagBiotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) ELISA bioactivity

Immobilized Cynomolgus / Rhesus macaque B7-2, His Tag (Cat. No. CD6-C52H5) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) with a linear range of 0.02-0.625 μg/mL (Routinely tested).

Biotinylated Human CTLA-4, Fc,AvitagBiotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) ELISA bioactivity

Immobilized Biotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Ipilimumab with a linear range of 0.2-2 ng/mL (Routinely tested).

Biotinylated Human CTLA-4, Fc,AvitagBiotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) ELISA bioactivity

Serial dilutions of Ipilimumab neutralizing antibody (1:2 serial dilutions, from 40 μg/mL to 0.019 μg/mL) were added into Human B7-1, Fc Tag (Cat. No. B71-H5259): Biotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.

Biotinylated Human CTLA-4, Fc,AvitagBiotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) ELISA bioactivity

Serial dilutions of Ipilimumab neutralizing antibody (1:2 serial dilutions, from 40 μg/mL to 0.019 μg/mL) were added into Human B7-2, Fc Tag (Cat. No. CD6-H5257): Biotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.

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