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Your Position: Home > Kits > PD-1 > EPH-V1

ELISA Assay Kit for Anti-PD-1 h-mAb in Human Serum

For research use only.

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  • Background
    How a therapeutic antibody is metabolized in the body is pertinently relevant to its efficacy. Therefore, pharmacokinetics study is an important part of the drug development. PD-1 is probably the most targeted molecule in today’s pharmaceutical industry, thanking to the clinical success of Keytruda® and Opdivo®. Many investigational anti-PD-1 mAbs are being developed. There is a growing need for a standard assay that can be used to facilitate the study of their pharmacokinetics.
  • Reconstitution
    See Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    Upon receipt, please store all items at -20°C.

    After reconstitution, the stock solution should be kept at -80°C.

  • Assay Principles
    The enclosed ELISA assay kit for anti-PD-1 h-mAb in human serum is based on an enzyme immunoassay (ELISA) between recombinant PD-1 protein (ECD) and a biotinylated anti-PD-1 monoclonal antibody. The method employs the principle of competitive ELISA, allowing quantification of monoclonal anti-PD-1 antibodies in human serum.

    The assay only involves the following steps:

    1. Coat the plate with human PD-1;

    2. Add the mixture of your sample and biotinylated anti-PD-1 antibody;

    3. Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate;

    4. Record the OD readings and analyze the serum concentration.

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