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Your Position: Home > Immune Checkpoint Proteins > CD27 Ligand > Human CD27 Ligand / CD70 Protein, Mouse IgG2a Fc Tag, low endotoxin

Human CD27 Ligand / CD70 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
  • Source
    Human CD27 Ligand, Mouse IgG2a Fc Tag, low endotoxin (CDL-H525a) is expressed from human 293 cells (HEK293). It contains AA Gln 39 - Pro 193 (Accession # P32970-1).
    Predicted N-terminus: Glu
  • Molecular Characterization
    CD27 Ligand(Gln 39 - Pro 193) P32970-1

    This protein carries a mouse IgG2a Fc tag at the N-terminus.

    The protein has a calculated MW of 44.0 kDa. The protein migrates as 50-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD27 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525a) SDS-PAGE gel

Human CD27 Ligand, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Bioactivity
Human CD27 Ligand, Mouse IgG2a Fc Tag, low endotoxinHuman CD27 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525a) ELISA bioactivity

Immobilized Human CD27 (Cat. No. CD7-H522b) at 2 μg/mL (100 μL/well) can bind Human CD27 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525a) with a linear range of 0.2-6 ng/mL (QC tested).

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