Login Register
icon_bulk_orderBulk order Acrobiosystems for ChineseChinese
Recombinant Proteins ClearClear history
Recombinant Proteins CloseClose
Browse Products By Molecule Name a-zA-Z
2019-nCoV | CD24 | CD73 | Trimer | CD19 | PD-1
0
There is no goods in the shopping cart !
My Cart
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > All Other Proteins > Siglec-2 > SI2-H525a

Human Siglec-2 / CD22 Protein, Llama IgG2b Fc Tag, low endotoxin

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    CD22,SIGLEC2,BL-CAM,SIGLEC-2,Siglec2,SIGLEC2FLJ22814
  • Source
    Human Siglec-2, Llama IgG2b Fc Tag, low endotoxin (SI2-H525a) is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Arg 687 (Accession # P20273-1).
    Predicted N-terminus: Asp 20
    Request for sequence
  • Molecular Characterization
    Online(Asp 20 - Arg 687) P20273-1

    This protein carries a llama IgG2b Fc tag at the C-terminus.

    The protein has a calculated MW of 103.0 kDa. The protein migrates as 120-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Siglec-2, Llama IgG2b Fc Tag, low endotoxin (Cat. No. SI2-H525a) SDS-PAGE gel

Human Siglec-2, Llama IgG2b Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Siglec-2, Llama IgG2b Fc Tag, low endotoxinHuman Siglec-2, Llama IgG2b Fc Tag, low endotoxin (Cat. No. SI2-H525a) ELISA bioactivity

Immobilized Human Siglec-2, Llama IgG2b Fc Tag, low endotoxin (Cat. No. SI2-H525a) at 2 μg/mL (100 μL/well) can bind Anti-Human CD22 with a linear range of 4-63 ng/mL (QC tested).

Protocol
Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
  • Background
    B-cell receptor CD22 is also known as Sialic acid-binding Ig-like lectin 2 (Siglec-2), B-lymphocyte cell adhesion molecule (BL-CAM), T-cell surface antigen Leu-14, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. CD22 mediates B-cell B-cell interactions, and may be involved in the localization of B-cells in lymphoid tissues. Siglec-2 / CD22 binds sialylated glycoproteins, one of which is CD45. Siglec2 / CD22 plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
  • References

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

Order Details

Price(USD) : $350.00

Price(USD) : $2310.00

Add to Cart
Bulk Inquiry Customize Product

Promotion & Exhibitions

  • PEGS Boston 2019
  • PepTalk 2019

Drug Development Status

  • Number of Launched Drugs：2 Details
  • Number of Drugs in Clinical Trials：33 Details
  • Latest Research Phase：Approved

Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)
Show All Distributors

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
US: +1 800-810-0816 (Toll Free)
AP: +86 400-682-2521
Email
Email
info@acrobiosystems.com
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA
linkedin twitter facebook

Subscribe newsletter

Leave a message

Copyright © 2017 ACROBiosystems. All Rights Reserved
中文网站|Related Links|Privacy Policy|Terms and Conditions|Legal|Sitemap