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Your Position: Home > All Other Proteins > Apolipoprotein A-II > APA-H51H6

Human Apolipoprotein A-II / APOA2 Protein, His Tag

  • Synonym
    Apolipoprotein A-II,APOA2,Apo-AII,ApoA-II,Apolipoprotein A2
  • Source
    Human Apolipoprotein A-II, His Tag (APA-H51H6) is expressed from E.coli cells. It contains AA Ala 19 - Gln 100 (Accession # P02652-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Ala 19 - Gln 100) P02652-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 11.3 kDa. The protein migrates as 11 kDa and 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Apolipoprotein A-II, His Tag (Cat. No. APA-H51H6) SDS-PAGE gel

Human Apolipoprotein A-II, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    Apolipoprotein A-II(APO-A2) stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. It is produced by the liver and is involved in cholesteryl ester formation and cholesterol transport from tissues to the liver. Recent studies have established the interaction between APOA2 -256T>C polymorphism and dietary saturated fatty acids intake in relation to obesity on healthy individuals. Significant interaction between APOA2-saturated fatty acids intake and body mass index (BMI). The result of this study confirmed the interaction between APOA2 -256T>C polymorphism and SFAs intake with BMI in type 2 diabetic patients.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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