This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 37.6 kDa. The protein migrates as 35-37 kDa under reducing (R) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH7.5 with glycerol as protectant.
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Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS-CoV-2 Papain-like Protease, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Measured by its ability to convert the substrate Dabcyl–FRLKGGAPIKGV–Edans.The specific activity is >2500 pmol/min/mg (QC tested).
Authors: Mellott DM, Tseng CT, Drelich A, et al.
Journal: ACS chemical biology 2021
Application: Cell-based assay
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