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Your Position: Home > All Other Proteins > SIRP alphaV8 > SI8-H82W6

Biotinylated Human SIRP alphaV8 / CD172a Protein, His,Avitag™

  • Synonym
    SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1
  • Source
    Biotinylated Human SIRP alpha V8, His,Avitag (SI8-H82W6) is expressed from human 293 cells (HEK293). It contains AA Glu 31- Arg 369 (S44L, S50T, I52T, H54R, V57A) (Accession # ATD50864.1).
    Predicted N-terminus: Glu 31
  • Molecular Characterization
    Online(Glu 31 - Arg 369 (S44L, S50T, I52T, H54R, V57A)) ATD50864.1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 40.8 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human SIRP alphaV8, His,Avitag™ (Cat. No. SI8-H82W6) SDS-PAGE gel

Biotinylated Human SIRP alphaV8, His,Avitag™ on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human SIRP alphaV8, His,Avitag™Biotinylated Human SIRP alphaV8, His,Avitag™ (Cat. No. SI8-H82W6) ELISA bioactivity

Immobilized Human CD47, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-H52A5) at 2 μg/mL (100 μL/well) can bind Biotinylated Human SIRP alphaV8, His,Avitag (Cat. No. SI8-H82W6) with a linear range of 0.02-1.25 μg/mL (QC tested).

  • Background
    Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $350.00

    Price(USD) : $1380.00

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