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This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 137.6 kDa. The protein migrates as 150-180 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS-CoV-2 Spike Trimer, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of SARS-CoV-2 Spike Trimer, His Tag (Cat. No. SPN-C52Hb) is more than 90% and the molecular weight of this protein is around 551-609 kDa verified by SEC-MALS.
Immobilized SARS-CoV-2 Spike Trimer, His Tag (Cat. No. SPN-C52Hb) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-3 ng/mL (QC tested).
Immobilized SARS-CoV-2 Spike Trimer, His Tag (Cat. No. SPN-C52Hb) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) with a linear range of 0.1-2 ng/mL (Routinely tested).
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer, His Tag (Cat. No. SPN-C52Hb) with a linear range of 0.4-13 ng/mL (Routinely tested).
Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer, His Tag (Cat. No. SPN-C52Hb) with a linear range of 0.1-13 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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