This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus followed by an Avi tag.
The protein has a calculated MW of 53.5 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE).
>80% as determined by SDS-PAGE.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Contact us for customized product form or formulation.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human GPRC5D, His,Avitag&Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 80%.
Anti-GPRC5D Antibody captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Biotinylated Human GPRC5D, His,Avitag&Flag Tag (Cat. No. GPD-H82D6) with an affinity constant of 0.112 μM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
Loaded Biotinylated Human GPRC5D, His,Avitag&Flag Tag (Cat. No. GPD-H82D6) on SA Biosensor, can bind Anti-GPRC5D Antibody, Human IgG4 with an affinity constant of 1.14 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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