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Your Position: Home > IgG kits > Spike RBD > RAS-T088

Mouse Anti-SARS-CoV-2 (BA.2) Antibody IgG Titer Serologic Assay Kit (Spike RBD)

For research use only.
IDComponentsSize
RAS088-C01Pre-coated SARS-CoV-2 Spike RBD (BA.2) Microplate1 plate
RAS088-C02Positive Control100 μL
RAS088-C03Negative Control100 μL
RAS088-C04HRP-Goat anti-Mouse IgG10 μg
RAS088-C0510xWashing Buffer50 mL
RAS088-C06Dilution Buffer50 mL
RAS088-C07Substrate Solution12 mL
RAS088-C08Stop Solution7 mL
  • Background
    A more contagious subvariant of omicron, known as BA.2, is spreading across the globe and could soon become the dominant version of Covid-19.Its now the top variant in at least 18 countries and rapidly spreading, representing 35% of all new cases that have been genetically sequenced worldwide, up from 10 countries and 21% of cases the week before, according to new data from the World Health Organization. In the U.S, BA.2 currently makes up 3.8% of genetically sequenced Covid cases, according to the Centers for Disease Control and Prevention.To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure IgG antibodies against the mutants is in urgent need.
  • Application
    This Kit is developed for qualitative detection or titer measurement of Anti-SARS-CoV-2 (BA.2) Antibody IgG (Spike RBD) in mouse serum.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Mouse serum by SARS-CoV-2 Spike RBD. The Kit consists of Pre-coated with SARS-CoV-2 Spike RBD (BA.2) Microplate , an Positive Control, an Negative Control, an HRP-Anti-Goat anti mouse IgG secondary antibody.

    Your experiment will include 4 simple steps:

    a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.

    b) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

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Price(USD) : $650.00

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