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Your Position: Home > Cell Grade Beads > CD3 & CD28 > MBS-C001

ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF FiledDMF

The Magnetic Stand (Cat.No. MB-01 & Cat.No. MB-02) can be used in conjunction with Beads.

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Materials Provided
Product Size Amount
ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed 2.5 mg 2.5 × 107 beads
10 mg 1 × 108 beads
  • Background
    ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed are uniform 5.5 μm of magnetic beads coated with an optimized mixture of mouse monoclonal antibodies against the CD3 and CD28, mimicking in vivo stimulation by APCs.
  • Application
    ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed is intended for the in vitro isolation, stimulation and expansion of purified T cell populations of, for example, CD3+T cells, CD4+ T cells, CD8+ T cells or human PBMC in early preclinical stage.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Endotoxin
    Less than 2EU/mg by the LAL method.
  • Reconstitution
    See Certificate of Analysis (CoA) for detailed instruction.
  • Storage

    The product is shipped at ambient temperature. Upon receipt, store it immediately at -20°C or below for long term storage. This product is stable after storage at:
    1 -20°C or below for 5 years in lyophilized state from date of receipt;
    2. 2-8°C for 12 months under sterile conditions after reconstitution.

    Please avoid repeated freeze-thaw cycles after reconstitution.

  • Important Note
    This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
Typical Data
TYPICAL DATA

Fig.1. Activation of the purified human T Cells.The  human T cells were stimulated with  ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade(Cat. No. MBS-C001) for 24hrs, and the activation was assessed by measuring expression of both activation markers CD25 and CD69 expression on the T cells surface by stanning with  PE labeled anti-human CD25 antibody and FITC labeled anti-human CD69 antibody respectively (QC tested).

TYPICAL DATA

Fig.2. Purified human T Cells expansion.The human T cells  were labeled with carboxy fluorescein succinimidyl ester (CFSE) and stimulated with  ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade(Cat. No. MBS-C001), and then the proliferation of the T cells was assessed with CFSE dilution assay  by  flow cytometry on day 5 after stimulation (QC tested).

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