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Your Position: Home > Antigen kits > Spike Trimer > RAS-A111

SARS-CoV-2 Spike Trimer (BA.1) Specific ELISA Kit (High Sensitivity)

For research use only.
IDComponentsSize
RAS111-C01 Pre-coated Anti-SARS-CoV-2 Spike Trimer Antibody Microplate1 plate
RAS111-C02SARS-CoV-2 Spike Trimer (BA.1)10 μg
RAS111-C03Biotin-Anti-SARS-CoV-2 Spike Trimer Antibody200 μL
RAS111-C04Streptavidin-HRP10 μg
RAS111-C0510xWashing Buffer 50 mL
RAS111-C06Dilution Buffer50 mL
RAS111-C07Substrate Solution12 mL
RAS111-C08Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 Spike protein is urgently needed to accelerate the development of COVID-19 vaccines.
  • Application
    The kit is developed for specific detection of SARS-CoV-2 Spike Protein (BA.1) in vaccine samples,witch can meet the needs of vaccine developers to establish antigen quantification methods for preclinical evaluation, vaccine production and quality control,and realize accurate quantification of vaccine antigen contents for COVID-19 vaccines of Omicron-specific boosters.

    It is for research use only.

  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike Protein. The kit consists of microplate pre-coated with Anti-SARS-CoV-2 Spike protein Antibody, an SARS-CoV-2 Spike Protein as Control, an biotin-Anti-SARS-CoV-2 Spike protein Antibody, HRP-Streptavidin and buffers.

    Your experiment will include 6 simple steps:

    a) All reagents were returned to room temperature(20°C-25°C) before use.

    b) Add your sample to the plate, take the SARS-CoV-2 Spike protein as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    c) Add a diluted Secondary antibody biotin-Anti-SARS-CoV-2 Spike protein Antibody to the plate. The Secondary antibody is diluted by Dilution Buffer.

    d) Add a diluted Streptavidin-HRP to the plate.

    e) Wash the plate and add TMB or other colorimetric HRP substrate. f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calc μLated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data
 Spike Trimer ELISA

Detection of Monoclonal SARS-CoV-2 Spike Trimer (BA.1) titer by sandwich-ELISA Assay.
Immobilized Anti-SARS-CoV-2 Spike Trimer Antibody can bind SARS-CoV-2 Spike Trimer (BA.1). Detection was performed using Biotin-Anti-SARS-CoV-2 Spike Trimer Antibody with sensitivity of 0.4 ng/mL (QC tested).

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ETD of in-stock products: 2 business days

Price(USD) : $780.00

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