Login Register
icon_bulk_orderBulk order Acrobiosystems for English
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Antigen kits > Spike Trimer > RAS-A115

SARS-CoV-2 Spike Trimer (Wild Type) Specific ELISA Kit (For Vaccine Development)

For research use only.
Materials Provided
IDComponentsSize
RAS115-C01Pre-coated Anti-SARS-CoV-2 Spike Trimer Antibody Microplate1 plate
RAS115-C02SARS-CoV-2 Spike Trimer (Wild Type)10 μg
RAS115-C03HRP-Anti-SARS-CoV-2 Spike Trimer Antibody20 μg
RAS115-C0410xWashing Buffer 50 mL
RAS115-C05Dilution Buffer50 mL
RAS115-C06Substrate Solution12 mL
RAS115-C07Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 Spike protein is urgently needed to accelerate the development of COVID-19 vaccines.
  • Application
    The kit is developed for specific detection of SARS-CoV-2 Spike Trimer (Wild Type) in vaccine samples, witch can meet the needs of vaccine developers to establish antigen quantification methods for preclinical evaluation, vaccine production and quality control,and realize accurate quantification of vaccine antigen contents for COVID-19 vaccines of Wild Type-specific boosters.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike Protein. The kit consists of microplate pre-coated with Anti-SARS-CoV-2 Spike protein Antibody, an SARS-CoV-2 Spike Protein as Control, an HPR-Anti-SARS-CoV-2 Spike protein Antibody,and buffers.

    Your experiment will include 5 simple steps:

    a) All reagents were returned to room temperature(20°C-25°C) before use.

    b) Add your sample to the plate, take the SARS-CoV-2 Spike protein as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    c) Add a diluted HRP-Anti-SARS-CoV-2 Spike Protein Antibody to the plate. The diluted by Dilution Buffer.

    d) Wash the plate and add TMB.

    e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data
SARS-CoV-2 Spike Trimer (Wild Type) Specific ELISA Kit (For Vaccine Development)SARS-CoV-2 Spike Trimer (Wild Type) Specific ELISA Kit (For Vaccine Development) (Cat. No. RAS-A115) ELISA bioactivity

Detection of Monoclonal SARS-CoV-2 Spike Trimer (Wild Type) titer by sandwich-ELISA Assay.
Immobilized Anti-SARS-CoV-2 Spike Trimer Antibody at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer (Wild Type). Detection was performed using HRP-Anti-SARS-CoV-2 Spike Trimer Antibody with sensitivity of 6.3 ng/mL (QC tested).

Comments (0)


ETD of in-stock products: 2 business days

Price(USD) : $780.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America: +1 800-810-0816 (Toll Free)
Asia & Pacific: +86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message