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Your Position: Home > Antigen kits > Spike RBD > RAS-A092

SARS-CoV-2 Spike RBD (B.1.617.2) Specific ELISA Kit (For Vaccine Development)

For research use only.
IDComponentsSize
RAS092-C01Pre-coated Anti-SARS-CoV-2 Spike RBD (B.1.617.2) Antibody Microplate1 plate
RAS092-C02SARS-CoV-2 Spike RBD (B.1.617.2)15 μg
RAS092-C03HRP-Anti-SARS-CoV-2 Spike RBD Antibody20 μg
RAS092-C0410xWashing Buffer 50 mL
RAS092-C05Dilution Buffer50 mL
RAS092-C06Substrate Solution12 mL
RAS092-C07Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 Spike protein is urgently needed to accelerate the development of COVID-19 vaccines.
  • Application
    This kit is developed for specific detection of SARS-CoV-2 Spike RBD (B.1.617.2) in vaccine samples, witch can meet the needs of vaccine developers to establish antigen quantification methods for preclinical evaluation, vaccine production and quality control,and realize accurate quantification of vaccine antigen contents for COVID-19 vaccines of Delta-specific boosters.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage & Shipping
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.

    Below is the Shipping Statement for Kit Products.

    Shipping Statement

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike RBD. The kit consists of microplate pre-coated with Anti-SARS-CoV-2 Spike RBD Antibody, an SARS-CoV-2 Spike RBD as Control, an HPR-Anti-SARS-CoV-2 Spike RBD Antibody,and buffers.

    Your experiment will include 5 simple steps:

    a) All reagents were returned to room temperature(20°C-25°C) before use.

    b) Add your sample to the plate, take the SARS-CoV-2 Spike RBD as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    c) Add a diluted HRP-Anti-SARS-CoV-2 Spike RBD Antibody to the plate. The diluted by Dilution Buffer.

    d) Wash the plate and add TMB.

    e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data Please refer to DS document for The assay protocol.
 Spike RBD ELISA

Detection of Monoclonal SARS-CoV-2 Spike RBD (B.1.617.2) titer by sandwich-ELISA Assay.
Immobilized Anti-SARS-CoV-2 Spike RBD (B.1.617.2) Antibody can bind SARS-CoV-2 Spike RBD (B.1.617.2). Detection was performed using HRP-Anti-SARS-CoV-2 Spike RBD Antibody with sensitivity of 3.75 ng/mL (QC tested).

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ETD of in-stock products: 2 business days

Price(USD) : $780.00

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