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Your Position: Home > Non SARS-Cov-2 Kits > AE > ABK-001

Chemiluminescent Substrate Solution (AE Marker)

ABK001-C01Trigger A (Oxidant solution)1 L
ABK001-C02Trigger B (Enhancer solution)1 L
  • Background
    The chemiluminescence substrate solution is a simple triggering reagent for acridinium ester (AE) fast light emission. Acridinium ester is the most commonly used for protein labeling and widely used in automated instruments because of its high sensitivity with detection limits in the attomole range. This product is suitable for acridinium ester labels on chemiluminescence technology, it is provided in two liquid buffer of Trigger A (Oxidant solution) and Trigger B (Enhancer solution) in each kit. When used, mix Trigger A and Trigger B in equal volume by 1:1, and light emission from acridinium esters is quite rapid and is essentially complete in <5s.
  • Application
    The chemiluminescence substrate solution is used to trigger acridinium ester labels to give a quite rapid light emission in chemiluminescence technology.
  • Storage
    4°C~30°C under sterile conditions for 12 months. Do not freeze. Product is shipped at ambient temperature.

When used the Chemiluminescent Substrate Solution (AE Marker) (Cat. No. ABK-001) in a sandwich MPCLIA Assay for detection of SARS-CoV-2, The Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (Cat. No. NUN-BM266) coupled Streptavidin-Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A006) was incubated with Acridine Ester-Labeled Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (AM224) (Cat. No. NUN-M224) and treated with SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5227) at increasing concentration, detection was performed using with sensitivity of 97.7 pg/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).

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