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Your Position: Home > Protein > Coagulation factor X > F10-C52H9

Cynomolgus Coagulation factor X / F10 Protein, His Tag (MALS verified)

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INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    Coagulation factor X,F10,factor 10,factor X,Stuart Factor,Stuart-Prower factor
  • Source
    Cynomolgus Coagulation factor X, His Tag(F10-C52H9) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Lys 488 (Accession # A0A2K5VHK1-1 ).
    Predicted N-terminus: Leu 24
    Request for sequence
  • Molecular Characterization
    Coagulation factor X Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 15.7 kDa and 30.3 kDa. The protein migrates as 19 kDa,48-53 kDa and 65-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 25 mM MES,150 mM NaCl,pH6.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Coagulation factor X SDS-PAGE

Cynomolgus Coagulation factor X, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Coagulation factor X MALS images

The purity of Cynomolgus Coagulation factor X, His Tag (Cat. No. F10-C52H9) is more than 90% and the molecular weight of this protein is around 52-70 kDa verified by SEC-MALS.

Report
  • Background
    As the only known physiological activator of thrombin, Factor X is a vitamin K-dependent plasma protease that plays a pivotal role in blood coagulation.
  • Clinical and Translational Updates

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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