|EP141-C01||High-bind Plate||1 plate|
|EP141-C02||Human VEGF R2||50 μg|
|EP141-C03||Biotinylated Human VEGFA||10 μg|
|EP141-C04||Anti-VEGF Neutralizing Antibody||20 μg|
|EP141-C06||Coating Buffer||12 mL|
|EP141-C07||10xWashing Buffer||50 mL|
|EP141-C08||Blocking Buffer||50 mL|
|EP141-C09||Substrate Solution||24 mL|
|EP141-C10||Stop Solution||7 mL|
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human VEGF R2.
b) Add your molecule of interest to the tests.
c) Add human VEGF165-Biotin to bind the coated VEGF R2.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to VEGF165: VEGF R2 binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF VEGFA[Biotinylated]: VEGF R2 BINDING BY ANTI-VEGFA NEUTRALIZING ANTIBODY
Serial dilutions of Anti-VEGFA Neutralizing antibody (Catalog # EP141-C04) (1:1 serial dilution, from 20 μg/mL to 0.01μg/mL) was added into Biotinylated VEGFA: VEGF R2 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $650.00
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