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Your Position: Home > Beads > Mouse IgG > MPC-A003

Anti-Mouse IgG-coupled Magnetic Beads (recommended for MPCLIA)

The Magnetic Stand (Cat.No. MB-01 & Cat.No. MB-02) can be used in conjunction with Beads.

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Materials Provided
ItemsDetails
Detection MethodChemiluminescence
Product TypeMagnetic Beads (Anti-Mouse IgG)
Quantity Size10mg / 100mg
Physical Appearancelyophilized powder mixture
Particle size2.8 µm
Beads SurfaceHydrophilic
Amount of Coupled ProteinAbout 133 pmol (20 μg) Monoclonal Rabbit anti-mouse IgG antibody / mg Beads
Binding Capacity2-5ug mouse antibody or Mouse IgG Fc tag proteins / mg beads
Emission WavelengthMeasured relative light units (RLU) at 430 nm
  • Background
    The Anti-Mouse IgG-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal Rabbit anti-mouse IgG antibody. The beads can be used to capture the mouse IgG in Chemiluminescence procedures. The antibody reacts with the heavy and light chain of mouse IgG. It can recognizes mouse IgG1, IgG2a, IgG2b and it binds IgG3 with a lower reactivity. It recognizes rat IgG and has no cross-reactivity with chicken IgY, goat IgG, human IgG, and rabbit IgG. The Anti-Mouse IgG-coupled Magnetic Beads is easy to capture the mouse IgG, and the bounded protein have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
  • Application
    The Anti-Mouse IgG-coupled Magnetic Beads is used to capture the mouse IgG, it can combination with Acridinium ester markers in chemiluminescence technology, The Acridinium ester markers such as Streptavidin-Acridinium ester can capture the biotinylated proteins or molecules, this allows detection of antigen and antibody binding or antibody screening.
    *The Isotype control (Cat. No. SMB-B01) is sold separately.
  • Reconstitution

    See Certificate of Analysis (CoA) for detailed instruction.

  • Storage
    Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
    Bioactivity-MPCLIA
     Mouse IgG MPCLIA

    Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (Cat. No. PD1-H82E4) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg /Test), incubated with 100 μL /Test of Human PD-L1 Protein, Mouse IgG1 Fc Tag (Cat. No. PD1-H52A3) at increasing concentration coupled to Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) (10 μg beads/Test). Detection was performed with sensitivity of 7.8 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).

     Mouse IgG MPCLIA

    The MPCLIA assay shows that Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) is stable at 37°C for 10 days.

     Mouse IgG MPCLIA

    The MPCLIA assay shows that Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) is stable after freezing and thawing 3 times.

     Mouse IgG MPCLIA

    The MPCLIA assay shows that Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) is stable at 37°C for 10 days.

    • Clinical and Translational Updates

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    Price(USD) : $116.00

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