This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 50 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human B7-1, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human B7-1, Fc Tag (HPLC-verified)(Cat.# B71-H5259) was greater than 95% as determined by SEC-HPLC.
Immobilized Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4 (Cat. No. CT4-H82E4) with a linear range of 50-500 ng/mL (Routinely tested).
Authors: G Aravindhan, et al.
Journal: BBA-GEN SUBJECTS 2018
Authors: Grzegorz B. Gmyrek, et al.
Journal: Cell Immunol 2017
Application: Binding assays
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Price(USD) : $260.00
Price(USD) : $1760.00
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