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Your Position: Home > Immune Checkpoint Proteins > B7-1 > Human CD80 B7-1 Protein, With C-Fc Tag

Human B7-1 / CD80 Protein, Fc Tag (HPLC-verified)

  • Synonym
    CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7
  • Source
    Human B7-1, Fc Tag (HPLC-verified) (B71-H5259) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # NP_005182.1).
    Predicted N-terminus: Val 35
  • Molecular Characterization
    B7-1(Val 35 - Asn 242) NP_005182.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 50 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) SDS-PAGE gel

Human B7-1, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Human B7-1, Fc Tag (HPLC-verified) (Cat. No. ) HPLC images

The purity of Human B7-1, Fc Tag (HPLC-verified)(Cat.# B71-H5259) was greater than 95% as determined by SEC-HPLC.

Bioactivity-ELISA
Human B7-1, Fc Tag (HPLC-verified)Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) ELISA bioactivity

Immobilized Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4 (Cat. No. CT4-H82E4) with a linear range of 50-500 ng/mL (Routinely tested).

Human B7-1, Fc Tag (HPLC-verified)Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) ELISA bioactivity

Immobilized Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) at 2 μg/mL ( 100 μl/well ) can bind Human CTLA-4, Strep Tag (Cat. No. CT4-H5284) with a linear range of 1-20 ng/mL (QC tested).

Human B7-1, Fc Tag (HPLC-verified)Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) ELISA bioactivity

Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, His Tag, Fc Tag (Cat. No. PD1-H82F3) with a linear range of 0.02-1.25 μg/mL (Routinely tested).

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