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Three Pre-Stained Color
Neat, Bright and Well-distributed Bands
More Accurate Molecular Weight Position
The product is shipped with blue ice. Upon receipt, store it immediately at -20°C for long term storage.
This product is stable after storage at:
-20°C for up to three years or 4°C for up to two months.
1. Thaw the product at room temperature for a few minutes to dissolve precipitated solids. Do not boil!
2. Mix gently, but thoroughly, to ensure the solution is homogeneous.
3. Load the following volumes of the product on an SDS-PAGE:
– 3-5 μL per well for mini gel
– 5-10 μL per well for large gel
Use the same volumes for Western blot. The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. The loading volume should be doubled for 1.5 mm thick gels.
| Number of Bands | 7 |
| Size Range | 40 to 275 kDa |
| Stain Type | 3 colors: Blue, Orange, Green |
| Molecular Weight | 275, 180, 130, 100, 72, 50, 40 kDa |
| Quantity | 2 x 250 ul, 10 x 250 ul |
| System Type | SDS-PAGE, Western Blot |
1. This product has been prepared in 1× SDS-PAGE loading buffer and can be used directly without boiling, diluting and adding reducing agent.
2. Longer transfer times or higher transfer voltages may be required for Western blot of large (>100 kDa) proteins.
3. Don’t add SDS to transfer buffer. If SDS must be used, the concentration should not exceed 0.02-0.04%.
4. In low-percentage gels (< 10 %), the low-molecular weight proteins in the ladder may migrate with the dye front.
5. Pre-stained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. See the table provided for migration patterns in different electrophoresis conditions.
6. For your safety and health, please wear a lab coat and disposable gloves.
SDS-PAGE band profile of the Star Ribbon Pre-stained Protein Marker
The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.
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