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Your Position: Home > Protein > CD2 > CD2-H5226

Human CD2 / SRBC Protein, His Tag (MALS verified)

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  • Synonym
    CD2,SRBC,LFA-2,T11
  • Source
    Human CD2, His Tag(CD2-H5226) is expressed from human 293 cells (HEK293). It contains AA Lys 25 - Asp 209 (Accession # AAH33583).
    Predicted N-terminus: Lys 25
  • Molecular Characterization
    CD2 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 22.3 kDa. The protein migrates as 33-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD2 SDS-PAGE

Human CD2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD2 MALS images

The purity of Human CD2, His Tag (Cat. No. CD2-H5226) is more than 90% and the molecular weight of this protein is around 23-35 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD2 ELISA

Immobilized Human CD58, Fc Tag (Cat. No. LF3-H5256) at 5 μg/mL (100 μL/well) can bind Human CD2, His Tag (Cat. No. CD2-H5226) with a linear range of 39-312 ng/mL (QC tested).

Bioactivity-BLI
 CD2 BLI

Loaded bind Human CD58, Fc Tag (Cat. No. LF3-H5256) on Protein A Biosensor, can bind Human CD2, His Tag (Cat. No. CD2-H5226) with an affinity constant of 0.49 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 CD2 BLI

Loaded bind Human CD58, Fc Tag (Cat. No. LF3-H5256) on AHC Biosensor, can bind Human CD2, His Tag (Cat. No. CD2-H5226) with an affinity constant of 0.57 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    T-cell surface antigen CD2 is also known as Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5 and SRBC, is a single-pass type I membrane protein found on the surface of T cells and natural killer (NK) cells. CD2 is a member of the immunoglobulin superfamily. CD2 / SRBC contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD2 / SRBC interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3 / CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. In addition to its adhesive properties, CD2 also acts as a co-stimulatory molecule on T and NK cells. CD2 is a specific marker for T cells and NK cells, and can therefore be used in immunohistochemistry to identify the presence of such cells in tissue sections.
  • Clinical and Translational Updates

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