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Your Position: Home > Cell > Alpha-synuclein > CHEK-ATP085

HEK293/Human Alpha-synuclein (GFP) Stable Cell Line

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  • Description
    HEK293/Human Alpha-synuclein (GFP) Stable Cell Line
  • Application

    α-Synuclein PFF induced intracellular aggregation assay

  • Growth Properties
  • Selection Marker
    Hygromycin B (250 μg/mL)
  • Culture Medium
    DMEM medium + 10% FBS
  • Freeze Medium
    10% DMSO + 90% FBS
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
  • Sterility Testing
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Receptor Assay
 Alpha-synuclein FACS

FACS analysis of Alpha-synuclein (GFP) on HEK293/Human Alpha-synuclein (GFP) Stable Cell Line.
HEK293/Human Alpha-synuclein (GFP) Stable Cell Line was red line, negative control HEK293 cells was grey line (QC tested).

Bioactivity-Bioactivity CELL BASE
 Alpha-synuclein CELL

HEK293/Human Alpha-Synuclein (GFP) Stable Cell Line (Cat. No. CHEK-ATP085) were transduced with Human Alpha-Synuclein Pre-formed Fibrils, His Tag and Human Alpha-Synuclein, His Tag (Cat. No. ALN-H52H8) respectively. The fluorescence of GFP-Alpha-Synuclein (Green) and DAPI (Blue) were detected by confocal microscope. A. Lipo2000 transduction. B. Lipo2000 and Human Alpha-Synuclein, His Tag transduction. C. Lipo2000 and Human Alpha-Synuclein Pre-formed Fibrils, His Tag transduction. Scale bars, 50 μm (Routinely tested).

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  • Background
    Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]
  • Permits & Restrictions
    This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $3500.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:20 Details
  • Latest Research Phase:Phase 3 Clinical

Datasheet & Documentation

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