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Your Position: Home > Cell > FcRn (FCGRT & B2M) > CHEK-ATP132

HEK293/FcRn (FCGRT & B2M), GFP Tag Stable Cell Line

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  • Description
    Human FcRn (FCGRT&B2M), GFP Tag-HEK293 cells stably expressing exogenous FCGRT ( AA sequence : Ala 24 - Ala 365, Protein Acc.# : P55899-1 ) & B2M ( AA sequence : Ile 21 - Met 119, Protein Acc.# : P61769-1 ). At the same time, GFP protein as a marker was expressed in HEK293/FcRn (FCGRT & B2M), GFP Tag Cells for screening positive cells by FACS.
  • Application

    Binding assay by FACS and cell based ELISA.

  • Growth Properties
    Adherent
  • Selection Marker
    Zeocin (25 μg/mL) + Puromycin (5 μg/mL)
  • Culture Medium
    DMEM medium +10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
    Negative
  • Sterility Testing
    Negative
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Bioactivity
 FcRn (FCGRT & B2M) FACS

Fig. FACS analysis of FcRn (FCGRT & B2M) on HEK293/FcRn (FCGRT & B2M), GFP Tag Stable Cell Line.
HEK293/FcRn (FCGRT & B2M), GFP Tag Stable Cell Line was red line, negative control HEK293 cells was grey line(QC tested).

Please contact us if you are interested in related cell pool service.

  • Background
    FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
  • License Disclosure
    This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:3 Details
  • Number of Drugs in Clinical Trials:3 Details
  • Latest Research Phase:Approved

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