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HEK293/Human Claudin-1 Stable Cell Line

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  • Description
    The HEK293/Human Claudin-1 Stable Cell Line was engineered to express the receptor full length human Claudin-1 (Gene ID: 9076), used to mimic cancer target cells. Surface expression of Human Claudin-1 was confirmed by flow cytometry.
  • Application

    • Useful for cell-based Claudin-1 binding assay

  • Growth Properties
  • Selection Marker
    Puromycin (2 μg/mL)
  • Culture Medium
    DMEM + 10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
  • Sterility Testing
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Receptor Assay
 Claudin-1 FACS

Expression analysis of human Claudin-1 on HEK293/Human Claudin-1 Stable Cell Line by FACS.
Cell surface staining was performed on HEK293/Human Claudin-1 Stable Cell Line or negative control cell using
anti-human Claudin-1 antibody followed by staining with FITC anti-mouse IgG Fc Antibody.

Passage Stability
 Claudin-1 PASSAGE

Passage stability analysis of receptors expression by FACS.
Flow cytometry surface staining of human Claudin-1 on HEK293/Human Claudin-1 Stable Cell Line demonstrates consistent mean fluorescent intensity across across passage 6-21.

Please contact us if you are interested in related cell pool service.

  • Background
    Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]
  • Permits & Restrictions
    This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $3500.00

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