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| Assay Type | Competitive-ELISA |
| Analyte | Gentamicin |
| Format | 96T |
| Regulatory Status | RUO |
| Sensitivity | 0.25 ng/mL |
| Standard Curve Range | 0.25 ng/mL-8 ng/mL |
| Assay Time | 1 hr 20 min |
| Suitable Sample Type | For the detection and quantitative determination of Gentamicin residues in plasmid DNA raw materials for cell and gene therap. |
| Sample volume | 50 μL |
| ID | Components | Size |
| RES-A025-C01 | Gentamicin Coated Plate | 1plate |
| RES-A025-C02 | Gentamicin Standard | 1.8mL |
| RES-A025-C03 | Anti-Gentamicin Antibody | 6mL |
| RES-A025-C04 | 1×Dilution Buffer | 50mL |
| RES-A025-C05 | 20xWashing Buffer | 50mL |
| RES-A025-C06 | Substrate Solution | 12mL |
| RES-A025-C07 | Stop Solution | 7mL |
The kit is developed for the detection of Gentamicin in drug products or semi-manufactures.
It is for research use only.
2. The reconstructed Gentamicin standard is stored at -70°C in at least 300ul per tube and cannot be frozen or thawed repeatedly.
3. Do not use reagents past their expiration date.
Serial dilutions of gentamicin (from 8 ng/mL to 0.25 ng/mL) was added into gentamicin: anti-gentamicin binding reactions. The assay was performed according to the protocol dscribed below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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