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Your Position: Home > Kits > Gentamicin > RES-A025

resDetect™ Gentamicin ELISA Kit

For research use only.

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Product Details
Assay TypeCompetitive-ELISA
Regulatory StatusRUO
Sensitivity0.25 ng/mL
Standard Curve Range0.25 ng/mL-8 ng/mL
Assay Time1 hr 20 min
Suitable Sample TypeFor the detection and quantitative determination of Gentamicin residues in plasmid DNA raw materials for cell and gene therap.
Sample volume50 μL
Materials Provided
RES-A025-C01Gentamicin Coated Plate1plate
RES-A025-C02Gentamicin Standard1.8mL
RES-A025-C03Anti-Gentamicin Antibody6mL
RES-A025-C041×Dilution Buffer50mL
RES-A025-C0520xWashing Buffer 50mL
RES-A025-C06Substrate Solution12mL
RES-A025-C07Stop Solution7mL
  • Background
    Residues of gentamicin are prone to occur in the production process of biological products, which can easily lead to abnormal reactions in the human body. Therefore, the residual amount of gentamicin in biological products or semi-finished products of biological products should be strictly controlled.
  • Application

    The kit is developed for the detection of Gentamicin in drug products or semi-manufactures.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. The kit is stable for 24 months from the date of manufacture if stored at 2°C to 8°C.

    2. The reconstructed Gentamicin standard is stored at -70°C in at least 300ul per tube and cannot be frozen or thawed repeatedly.

    3. Do not use reagents past their expiration date.

  • Assay Principles
    The Gentamicin quantitative detection kit adopts the indirect competitive ELISA method, and the pre-coated conjugated Gentamicin antigen on the microstrip competes with the residual Gentamicin in the sample to bind the enzyme-labeled anti-Gentamicin monoclonal antibody, and then uses a microplate reader to detect the absorbance value by adding TMB substrate, and the absorbance value is negatively correlated with the content of kanamycin in the sample. The kit takes only about one hour and 20 minutes to operate and has a linear range of 0.25 ng/mL to 8 ng/mL.
Typical Data Please refer to Ds document for the assay protocol.
 Gentamicin TYPICAL DATA

Serial dilutions of gentamicin (from 8 ng/mL to 0.25 ng/mL) was added into gentamicin: anti-gentamicin binding reactions. The assay was performed according to the protocol dscribed below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

  • Clinical and Translational Updates

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Price(USD) : $600.00

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