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Your Position: Home > Kits > ANGPTL3 > CEA-B089

ClinMax™ Human ANGPTL3 ELISA Kit, PRO

For research use only.

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Product Details
Assay TypeSandwich-ELISA
AnalyteANGPTL3
Format96T
ReactivityHuman
Sensitivity0.156 ng/mL
Standard Curve Range0.156 ng/mL-10 ng/mL
Assay Time2 hr 45 min
Suitable Sample TypeFor the quantitative determination of human ANGPTL3 in Cell Culture Supernatants, Plasma, Serum.
Sample volume50 uL
Materials Provided
IDComponentsSize
CEA089-B01Pre-coated Anti-ANGPTL3 Antibody Microplate1 plate
CEA089-B02Human ANGPTL3 Standard40 μg×2
CEA089-B03Biotin-Anti-ANGPTL3 Antibody Con. Solution100 μL
CEA089-B04Biotin-Antibody Dilution Buffer8 mL
CEA089-B05Streptavidin-HRP Con. Solution500 μL
CEA089-B06Streptavidin-HRP Dilution Buffer15 mL
CEA089-B0720× Washing Buffer50 mL
CEA089-B082×Sample Dilution Buffer15 mL×2
CEA089-B09Substrate Solution12 mL
CEA089-B10Stop Solution6 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human ANGPTL3 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-like facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of human ANGPTL3 in serum, plasma and cell culture supernates.

    It is for research use only.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
  • Assay Principles
     ANGPTL3 Assay Principles
Typical Data Please refer to DS document for the assay protocol.
 ANGPTL3 TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of ANGPTL3 is less than 0.156 ng/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 4 samples containing different ANGPTL3 concentrations were tested in one assay. Acceptable criteria: CV<10%.

 ANGPTL3 INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Five samples containing different concentrations of ANGPTL3 were tested in independent assays. Acceptable criteria: CV<15%.

 ANGPTL3 INTER-ASSAY STATISTICS
Recovery

Recombinant ANGPTL3 was spiked into 3 human serum samples, and then analyzed. The average recovery of ANGPTL3 for serum samples is 100.5%.

 ANGPTL3 RECOVERY
  • Background: ANGPTL3
    Angiopoietin-like 3, also known as ANGPTL3, is a protein that in humans is encoded by the ANGPTL3 gene. In human, ANGPTL3 is a determinant factor of HDL level and positively correlates with plasma HDL cholesterol.
  • Clinical and Translational Updates

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