Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Protein > Glycoprotein H&L > GHL-H5243

HHV-5 (strain AD169) Glycoprotein H&L, His Tag (MALS verified)

Order Now

  • Source
    HHV-5 (strain AD169) Glycoprotein H&L, His Tag(GHL-H5243) is expressed from human 293 cells (HEK293). It contains AA Val 31 - Arg 278 & Arg 24 - Leu 720 (Accession # P12824 & P16832).
    Predicted N-terminus: His
  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 109.5 kDa. The protein migrates as 110-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Glycoprotein H&L SDS-PAGE

HHV-5 (strain AD169) Glycoprotein H&L, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Glycoprotein H&L MALS images

The purity of HHV-5 (strain AD169) Glycoprotein H&L, His Tag (Cat. No. GHL-H5243) is more than 90% and the molecular weight of this protein is around 210-250 kDa verified by SEC-MALS.

 Glycoprotein H&L ELISA

Immobilized HHV-5 (strain AD169) Glycoprotein H&L, His Tag (Cat. No. GHL-H5243) at 1 μg/mL (100 μL/well) can bind Anti-HCMV gH [HCMV16], Human, IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

 Glycoprotein H&L ELISA

Immobilized Anti-HCMV gH [HCMV16], Human, IgG1 at 1 μg/mL (100 μL/well) can bind HHV-5 (strain AD169) Glycoprotein H&L, His Tag (Cat. No. GHL-H5243) with a linear range of 0.004-0.25 μg/mL (Routinely tested).

 Glycoprotein H&L SPR

Anti-HCMV gH [HCMV16] captured on Protein A Chip can bind HHV-5 (strain AD169) Glycoprotein H&L, His Tag (Cat. No. GHL-H5243) with an affinity constant of 1.49 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Human cytomegalovirus (HCMV), a prototypical beta-herpes virus, is a major cause of morbidity and mortality in immunocompromised transplant recipients and congenitally infected fetuses.Different HCMV glycoprotein complexes are capable of meditating entry through interaction with cell-specific receptors on a panel of host cells. The pentamer (gH/gL/UL128/UL130/UL131A) mediates HCMV entry into epithelial, endothelial, and myeloid cells by its binding to neuropilin 2 (Nrp2). The trimer (gH/gL/gO) can mediate infection of all cell types by binding to platelet-derived growth factor-alpha (PDGFRα). Both pentamer and trimer need to interact with the glycoprotein B (gB), the fusogenic protein, to trigger the virus and host cell membrane fusion.
  • Clinical and Translational Updates

Comments (0)

ETA of in-stock products: 2 business days

Price(USD) : $780.00

Price(USD) : $3600.00

Promotion & Exhibitions

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
+1 888-377-6111
1 Innovation Way, Newark, DE 19711, USA

Leave a message