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Your Position: Home > Kits > Pre-Fusion glycoprotein F0 > RAS-A178

HRSV Pre-Fusion glycoprotein F0(site V) Specific ELISA Kit (For Vaccine Development)

For research use only.

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RAS178-C01Pre-coated Anti-RSV-Pre-Fusion glycoprotein F0 (site V) Antibody Microplate1 plate
RAS178-C02Pre-Fusion glycoprotein F0 (RSV) Standard20 μg
RAS178-C03HRP-Anti-Fusion glycoprotein F0 (RSV) Antibody20 μg
RAS178-C0410xWashing Buffer 50 mL
RAS178-C052xDilution Buffer50 mL
RAS178-C06Substrate Solution12 mL
RAS178-C07Stop Solution7 mL
This product is still under development. Please contact us if you have interest in this product. We will accelerate the development process accordingly and reserve this product for you as request.
  • Background
    Respiratory syncytial virus (RSV) is a highly contagious virus causing severe infection in infants and the elderly. Various approaches are being used to develop an effective RSV vaccine. The RSV fusion (F) subunit, particularly the cleaved trimeric pre-fusion F, is one of the most promising vaccine candidates under development.

    A rapid and effective assay kit detecting the levels of HRSV Pre-Fusion glycoprotein F0 is urgently needed to accelerate the development of RSV vaccines.

  • Application

    This kit is developed for Specific quantitative detection of HRSV Pre-Fusion glycoprotein F0(site V) in samples.

    This kit can specifically identify HRSV (A) and HRSV (B) Pre-Fusion glycoprotein F0 by testing.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of RSV-Pre-Fusion glycoprotein F0 (site V). The kit consists of Pre-coated Anti-RSV-Pre-Fusion glycoprotein F0 (site V) Antibody Microplate and Pre-Fusion glycoprotein F0 (RSV) Standard,HRP-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody and buffers.

    Your experiment will include 5 simple steps:

    a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

    b) Add your sample to the plate, take the Pre-Fusion glycoprotein F0 (RSV) as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    c) Add the HRP-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody diluted by Dilution Buffer to the plate.

    d) Wash the plate and add TMB.

    e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

  • Clinical and Translational Updates

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