5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Mouse IgG1 Antibody Isotype Control (Cat. No. DNP-PM1) and positive control PE-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Cat. No. FM3-HPY53) respectively, and PE signal was used to evaluate the binding activity (QC tested).
Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse IgG2a Antibody Isotype Control (Cat. No. DNP-BLM487) with a linear range of 0.6-20 ng/mL (QC tested).
The purity of Human IgG1 (L234A, L235A) Kappa Isotype Control (mAb) (Cat. No. DNP-C18P1) is more than 95% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
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