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Adalimumab

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Part of Bioactivity data

ADB-Y19-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-Adalimumab Antibodies (mouse IgG1, Cat. No. ADB-Y19) captured on CM5 chip via anti-mouse antibodies surface, can bind human adalimumab with an affinity constant of 1.36 pM.

ADB-BY17-ELISA
Biotinylated Anti-Adalimumab Antibodies (recommended for PK/PD)Biotinylated Anti-Adalimumab Antibodies (recommended for PK/PD) (Cat. No. ADB-BY17) ELISA bioactivity

Detection of adalimumab by bridging ELISA in serum. Immobilized Anti-Adalimumab Antibodies (Cat. No. ADB-Y23) at 2 μg/mL, add increasing concentrations of adalimumab (10% human serum) and then add Biotinylated Anti-Adalimumab Antibodies (Cat. No. ADB-BY17) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 4.8 ng/mL.

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Background

Adalimumab is a human monoclonal antibody against TNF-alpha. It is produced by recombinant DNA technology using a mammalian cell expression system. It consists of 1330 amino acids and has a molecular weight of approximately 148 kilodaltons.

Clinical and Translational Updates

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